We offer genome editing services in cell lines for the most diverse research projects. We have developed streamlined gene knock-out protocols for standard cell lines. Highly customized approaches are developed together with the researchers to realize more complex genome editing projects such as knock-in, point mutations, tagging to name a few.
This is a list of our most frequently asked questions:
It depends on the kind of project. KO projects in easy-to-transfect diploid cells are usually accomplished in 6 to 10 weeks.
More complex projects may take up to 4-6 months.
Please come visit/contact us, we’ll be happy to answer your questions.
A CRISPR/Cas9 project is made of many different modules whose combination in a defined workflow depends on the final scientific goal. Please come visit/contact us, we’ll be happy to work out a customized quotation with you.
We usually do Western blot or Sanger sequencing. If you have different needs we can discuss.
No, we don’t. Instead we offer the generation of KO clones obtained with two different gRNAs.
For technical reasons we do not do primary cells, but we do ES cells. For other cell lines we offer a feasibility test (Cas9 delivery, clonability e promoter activity assays).
Please write to crispr-service[@]cogentech.it or call 02-574303804. We are located in building 9, first floor, open space lab.